The Blues are considered strong favourites for the tie and will be looking to progress into the next round.
Thomas Tuchel has made several changes to his Chelsea starting XI ahead of the clash.
Chelsea XI to face Chesterfield: Bettinelli; Hudson-Odoi, Christensen, Sarr, Hall; Ziyech, Kovacic, Saul, Pulisic; Lukaku, Werner
Chesterfield XI to face Chelsea: Loach, Kerr, Gunning, Croll, King, Weston, Oyeleke, Whittle, Khan, Kellermann, Tshimanga
Bench: Minter, Miller, Maguire, Asante, Mandeville, McCourt, Tyson, Grimes, Payne